Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Abstract Tripeptidyl-peptidase II (TPPII), is present in most eukaryotic cells. It cuts tripeptides from the N-terminus of peptides and is especially important for degrading peptides longer than 15 amino acids. TPPII also tailors long peptides into suitable substrates for the enzymes which transport and produce the peptides that MHC I present. Increased levels of TPPII have also been found in certain cancer cells, thus it is of interest to determine if TPPII could be used as a tumour marker.The aim of this study was to optimise and evaluate 3 different methods for quantifying TPPII. Western blot, enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions.

Concentration of protein in bovine milk is one of the most significant milk quality parameters, to a large extent determining the price for milk to the producer. Mastitis is a common disease among dairy cows, negatively affecting not only milk yield but also milk protein composition. Milk from mastitic cows tends to have lower cheese yield, negatively affected processability properties and sensory quality, due to changed protein quality and composition. Poorer milk protein quality would have an economically negative impact on dairy industry. Only few studies have investigated the short term effects of mastitis on milk protein composition.

The Bovine respiratory syncytial virus (BRSV) causes bronchiolitis and interstitial pneumonia, predominantly in calves, and is a major cause of bovine respiratory disease worldwide. In humans, BRSV is paralleled by the closely related Human respiratory syncytial virus (HRSV), an important cause of respiratory disease, most severe in infants.The clinical signs and pathology during RSV infection is caused, not only by the direct effects of viral replication, but also by the response of the host immune system. The immunopathology of RSV has long obfuscated our understanding of the disease, and development of effective treatment and vaccines will be very difficult until greater knowledge is gained.One of the components of the immune system that has come into focus in RSV research the last few years, is the Toll-like receptor 4 (TLR4). The TLR4 receptor is well known as the receptor that binds lipopolysaccaride (LPS), and initiates the host response to bacterial infection. Recently, it has been shown that the fusion protein of RSV also interacts with, and up-regulates the expression of, the TLR4 receptor.

An inflammatory reaction is induced after release of proinflammatory mediators such asinterleukin 1 and 6 and tumour necrosis factor ?. These mediators stimulate the liver tosuppress the syntheses of albumin and endure the syntheses of acute phase protein forinstance C-reactive protein. The aim of this paper was to perform a method validation on animmune turbidimetric assay to quantify C-reactive protein in canine serum at the laboratory atSkara Animals Hospital, Skara, Sweden. The validation involved evaluation of the assaylinearity, precision, stability and recovery.The method was proved to be linear for both TruLab control and Medinor control.

Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

Kosten har stor betydelse för den idrottande individens prestation och hälsa. Trots det är det få idrottsutövare som följer kostrekommendationerna för sin idrottsgren. Styrketränande individer får ofta i sig mer protein än de behöver och under de senaste åren har användningen av proteintillskott ökat. Ett för högt intag av protein kan vara skadligt för hälsan och kostsamt för såväl plånbok som miljö. Tidigare forskning har visat ett samband mellan attityder och beteenden, vilket innebär att det är av intresse att undersöka individers attityder om man vill förändra deras beteende.

Syftet med den här litteraturstudien var att undersöka effekten av hög protein- och fettrik kost på styrketräning. De flesta dagliga matvaror innehåller oftast både protein och fett förutom rena kolhydratkällor, vilka har olika effekt på kroppen vid styrketräning. Styrketräning kan påverka proteinsyntesen, muskelhypertrofi, lipolys och fettoxidationen i kroppen. Dessa processer influeras av mängden protein eller fett som konsumeras, vilken typ av protein eller fett som konsumeras samt tidpunkten intaget sker i relation till styrketräning.Studier har visat att ett högt proteinintag i kombination med styrketräning kan leda till ökad muskelmassa och viktnedgång på grund av en ökad proteinsyntes, lipolys och fettoxidation. Tidigare forskning visar också att ett ökat fettintag stimulerar lipolys och fettoxidationen hos individer.

Cyanobacteria belong to the oldest organisms of our planet. They use photosynthesis to produce ATP and gain biomass from carbon dioxide. The cyanobacteria Nostoc punctiforme is a filamentous bacterium that consists of two different types of cells, vegetative cells and heterocysts. The type of cell it differentiates into depends on the media they grow in. In an ammonium-rich medium, the N.punctiforme consists of vegetative cells that differentiate into heterocysts when in the medium is changed to a low-concentration ammonium medium.

Som SPA-terapeuter kommer vi att arbeta mycket med huden och få mycket frågor på hur man tar hand om sin hud på bästa sätt. Därför vill vi studera samband mellan hud och näringsämnen i kosten för att se om kosten kan påverka hudtillståndet. Vår fråga är därför påverkas huden av fett, kolhydrater och protein i kosten?Vi undersöker tre av våra största näringsämnen vilka är kolhydrater, protein och fett genom att titta på vetenskapliga artiklar som har gjorts inom området..

The purpose with this literature study was to investigate the possibilities for improvement of forage protein in dairy cows with focus on feeding strategies and conservation of forage protein. The utilization of forage protein in dairy cows is often not that efficient as it could be. The feed crude protein consists of both true proteins and non protein nitrogen. The digestion of proteins in the rumen depends on the protein degradation rate and the ability of the microbes to synthesize microbial protein. The microbes need a balance between carbohydrates that provide them with energy and proteins that are degradable in the rumen.

Protein is an important component in various milk products and has a crucial role in the final quality. Excluding non-protein nitrogen, bovine milk contains about 3.3% protein whereas about 80% of this fraction consists of casein. There are four major members in the casein family: ?s1-casein, ?s2- casein, ?-casein and ?-casein. Proteolytic enzymes are the cause of degradation of the economically important milk proteins.

The purpose of this study was to examine whether urea could be an option as a nitrogen source for young cattle, the study was limited to risks related to feeding, growth and feed efficiency. Microbes in ruminants have the ability to convert nitrogen to protein, this has been highlighted by beef producers as they are in search of cheap protein feed resources. Urea can be a worthy source of nitrogen for growing young animals, because of its high nitrogen content and the low price. Studies have shown that the replacement of a portion of the total protein content by urea in diets with low content of crude protein, like corn silage, may improve animal growth and feed efficiency. Replacement of all the true protein with urea can result in reduced feed intake and microbial growth.